Research Institute for Photobiological Hydrogen Production

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  • Research Institute for Photobiological Hydrogen Production

H2 production in Bottle and Bag Color variation of photosynthetic bacteria

Research Projects

1. Photobiological Hydrogen Production

Key words: Nitrogenase-based H2 production, Cyanobacteria, Purple bacteria, Flexible H2 barrier plastic bioreactor

2. Purple bacteria

Key words: Purple bacteria, Reaction center and electron transport system, Genome analysis

3. Green sulfur and Helio bacteria,

Key words: Chlorobaculum tepidum, Inorganic sulfur metabolism, Electron transport, Helio bacteria

Publications

Photobiological Hydrogen Production

*Sakurai, H., Masukawa, H., Kitashima, M., Inoue, K. (2015) How Close We Are to Achieving Commercially Viable Large-Scale Photobiological Hydrogen Production by Cyanobacteria: A Review of the Biological Aspects Life, 2015, 5, 997-1018

* Masukawa, H., Sakurai, H., Hausinger, R.P., Inoue K. (2014) Sustained photobiological hydrogen production in the presence of N2 by nitrogenase mutants of the heterocyst-forming cyanobacterium Anabaena International journal of hydrogen energy, 39, 19444-19451

*Sakurai, H., Masukawa, H., Kitashima, M., Inoue, K. (2013) Photobiological hydrogen production: Bioenergetics and challenges for its practical application J Photochem Photobiol C: Photochemistry Reviews, 17:1窶・5.

* Masukawa, H., Kitashima, M., Inoue, K., Sakurai, H., Hausinger, R.P. (2012) Genetic Engineering of Cyanobacteria to Enhance Biohydrogen Production from Sunlight and Water. AMBIO, 41, 169-173

* Kitashima, S., Masukawa, H., Sakurai, H., Inoue, K. (2012) Flexible plastic bioreactors for photobiological hydrogen production by hydrogenase-deficient Cyanobacteria. Biosci. Biotechnol. Biochem., 76, 831-833

* Masukawa, H., Inoue, K., Sakurai, H., Wolk, CP., Hausinger, RP. (2010) Site-directed mutagenesis of the Anabaena sp. strain PCC 7120 nitrogenase active site to increase photobiological hydrogen production. Appl. Env. Microbiol. 76: 6741-6750.

* Sakurai, H., Masukawa, H. (2007) Promoting R amp; D in photobiological hydrogen production utilizing mariculture-raised cyanobacteria. Mar. Biotechnol. 9: 128-145.

Purple bacteria

*Nagashima KVP, Vermeglio A, Fusada N, Nagashima S, Shimada K, Inoue K (2014) Exchange and Complementation of Genes Coding for Photosynthetic Reaction Center Core Subunits among Purple Bacteria Journal of Molecular Evolution 79, 52-62

*Nagashima, S., Nagashima K. V. P. (2013) Evolution of photosynthesis suspected from genome comparisons in purple bacteria. In Beatty T. J. (Ed.), Genome Evolution of Photosynthetic Bacteria, pp151-178, Academic Press, Elsevier Inc.

*Nagashima, S., Nagashima K. V. P. (2013) Comparison of photosynthesis gene clusters retrieved from total genome sequences of purple bacteria. In Beatty T. J. (Ed.), Genome Evolution of Photosynthetic Bacteria, pp151-178, Academic Press, Elsevier Inc.

*Nagashima, S., Kamimura, A., Shimizu, T., Nakamura-Isaki, S., Aono, E., Sakamoto, K., Ichikawa, N., Nakazawa, H., Sekine, M., Yamazaki, S., Fujita, N., Shimada, K., Hanada, S., Nagashima, K.V. (2012) Complete Genome Sequence of Phototrophic Betaproteobacterium Rubrivivax gelatinosus IL144. J. Bacteriol. Jul;194:3541-2.

* Vermﾃｩglio A, Nagashima S, Alric J, Arnoux P and Nagashima KVP (2012) Photo-induced electron transfer in intact cells of Rubrivivax gelatinosus mutants deleted in the RC-bound tetraheme cytochrome: Insight into evolution of photosynthetic electron transport. Biochim. Biophys. Acta 1817, 689-96

*Kondo M, Iida K, Dewa T, Tanaka H, Ogawa T, Nagashima S, Nagashima KVP, Shimada K, Hashimoto H, Gardiner AT, Cogdell RJ and Nango M (2012) Photocurrent and electronic activities of oriented-His-tagged photosynthetic light-harvesting/reaction center core complexes assembled onto a gold electrode. Biomacromolecules 13, 432-438

*Nagashima S, Kamimura A, Shimizu T, Nakamura S, Aono E, Sakamoto K, Ichikawa N, Nakazawa H, Sekine M, Yamazaki S, Fujita N, Shimada K, Hanada S and Nagashima KVP (2012) Complete genome sequence of phototrophic beta-proteobacterium Rubrivivax gelatinosus IL144. J. Bacteriol. 194, 3541-3542

*Okubo T, Tsukui T, Maita H, Okamoto S, Oshima K, Fujisawa T, Saito A, Futamata H, Hattori R, Shimomura Y, Haruta S, Morimoto S, Wang Y, Sakai Y, Hattori M, Aizawa SI, Nagashima KVP, Masuda S, Hattori T, Yamashita A, Bao Z, Hayatsu M, Kajiya-Kanegae H, Yoshinaga I, Sakamoto K, Toyota K, Nakao M, Kohara M, Anda M, Niwa R, Jung-Hwan P, Sameshima-Saito R, Tokuda SI, Yamamoto S, Yamamoto S, Yokoyama T, Akutsu T, Nakamura Y, Nakahira-Yanaka Y, Takada Hoshino Y, Hirakawa H, Mitsui H, Terasawa K, Itakura M, Sato S, Ikeda-Ohtsubo W, Sakakura N, Kaminuma E and Minamisawa K (2012) Complete genome sequence of Bradyrhizobium sp. S23321: Insights into symbiosis evolution in soil oligotrophs. Microbes Environ. 27, 306-315

*Hirose S, Nagashima KVP, Matsuura K and Haruta S (2012) Diversity of purple phototrophic bacteria, inferred from pufM gene, within epilithic biofilm in Tama river, Japan. Microbes Environ. 27, 327-329・・011・・/p>

*Nagashima S, Shimada K, Vermﾃｩglio A, Nagashima KVP (2011) The cytochrome c8 involved in the nitrite reduction pathway acts also as electron donor to the photosynthetic reaction center in Rubrivivax gelatinosus. Biochim. Biophys. Acta 1807, 189-196

*Tsukatani Y, Nakayama N, Shimada K, Mino H, Itoh S, Matsuura K, Hanada S, Nagashima KVP (2009) Characterization of a blue-copper protein, auracyanin, of the filamentous anoxygenic phototrophic bacterium Roseiflexus castenholzii. Arch. Biochem. Biophys. 490, 57-62

Green sulfur and Helio bacteria

* Sakurai, H., Ogawa, T., Shiga, M., Inoue, K. (2010) Inorganic sulfur oxidizing system in green sulfur bacteria. Photosyn. Res. 104: 163-176.

* Ogawa, T. Furusawa, T.. Shiga, M., Seo, D., Sakurai, H., Inoue, K. (2010) Biochemical studies of a soxF-encoded monomeric flavoprotein purified from the green sulfur bacterium Chlorobaculum tepidum that stimulates in vitro thiosulfate oxidation. Biosci. Biotechnol. Biochem. 74: 771-780.

*Ohashi, S., Iemura, S., Okada, T., Itoh, N., Furukawa, S., Okuda, H., Ohnishi-Kameyama, M., Ogawa, T., Miyashita, H., Watanabe, T., Itoh, S., Oh-oka, K., Inoue, K., Kobayashi, M. (2010). An overview on chlorophylls and quinones in the photosystem I-type reaction centers. Photosyn. Res. 104: 305-319

*Ogawa T, Ogawa T, Furusawa, Nomura, R., Seo, D., Hosoya-Matsuda, N., Sakurai, H., Inoue, K. et al. (2008) SoxAX binding protein, a novel component of the thiosulfate-oxidizing multienzyme system in the green sulfur bacterium Chloribium tepidum. J Bacteriol. 190: 6097-6110

Japanese Patent

Title of Invention: Gas sampling device

Patent owners: Waseda University and Hidehiro Sakurai Inventors: Hidehiro Sakurai, Hajime Masukawa, Kazuhito Inoue, Masaharu Kitashima (No. 5294128, Date: 21th June, 2013)