Nitrogenase-based H2 Production by Cyanobacteria
N2 + 8 e- + 8 H+ + 16 ATP → H2 + 2 NH3 + 16 (ADP + Pi)
(In the absence of N2 ) 2 e- + 2 H+ + 4 ATP → H2 + 4 (ADP + Pi)
Nitrogenase-based H2 Production by Cyanobacteria
Construction of Nostoc sp. PCC 7422 ΔHupL mutant
Sequencing and disruption of the gene to create ΔHupL mutant of Nostoc sp. PCC 7422
(We thank Dr.C.P.Wolk for plasmids)
White fluorescent light
90 mmol photon m-2s-1 (PAR)
Accumulation of H2 to 30%
Energy conversion efficiency 3.7%(vs. PAR) 1.7%(vs. solar energy)
Improvement of nitrogenase
(Air) N2 + 8e- + 8H+ + 16ATP → H2 + 2HN3 + 16(ADP + Pi)
(Ar) 2e- + 2H+ + 4ATP → H2 + 4(ADP + Pi)