• 神奈川大学プロジェクト研究所
• 光合成水素生産研究所(理学部生物科学科井上研究室)
• Research Institute for Photobiological Hydrogen Production

櫻井　英博（さくらい　ひでひろ）・理学博士

光合成水素生産研究所客員教授

早稲田大学名誉教授

略歴

東京大学大学院化学系研究科(生物化学専門課程)博士課程修了、早稲田大学教育学部助手、専任講師、助教授、教授を経て、現在、早稲田大学名誉教授。 この間、早稲田大学環境保全センター所長、早稲田大学持続的未来研究所長、神奈川大学総合理学研究所客員教授(現在に至る)

研究テーマ

緑色硫黄細菌の光化学反応系と電子伝達系

ラン色細菌による光生物学的水素生産

光合成系の抗酸化的ストレス系テントキシンのATP合成酵素に対する作用機構

主要著書・論文

[最近の論文]

Masukawa, H., Inoue, K., Sakurai, H., Wolk, C. P. and Hausinger R. P. (2010)

Site-directed mutagenesis of the Anabaena sp. strain PCC 7120 nitrogenase active site to increase photobiological hydrogen production.Appl. Environ. Microbiol. 76, 6741-6750.

Kitashima, M., Masukawa, H., Sakurai, H. and Inoue, K. (2012)

Flexible plastic bioreactors for photobiological hydrogen production by hydrogenase-deficient cyanobacteria. Biosci. Biotech. Biochem. 76, 831-833.

Sakurai, H., Masukawa, H., Kitashima, M. and Inoue, K. (2013)

photobiological hydrogen production: Bioenergetics and challenges for its practical application. J. Photochem. Photobio. C: Photochem. Rev. 17, 1-25.

増川一、北島正治、櫻井英博、　井上和仁 (2012)

ラン藻の窒素固定酵素ニトロゲナーゼを利用した大規模な水素生産構想, pp.80-87.　微細藻類によるエネルギー生産と事業展開（竹山春子監修）CMC出版.

[研究開発構想の解説等]

櫻井英博、増川一.

シアノバクテリアによる光生物的水素生産－水素を一次エネルギーとして大規模生産を目指す－. 燃料電池 6 (No.2): 46-52 (2006)

増川一、櫻井英博、

シアノバクテリアを用いた水素生産． 遺伝, 11月号, 46-51 (2006)

櫻井英博、増川一．

ラン色細菌の光生物的水素生産　化石燃料代替エネルギーの開発を目指して．蛋白質核酸酵素48: 1824-1831 (2003)

櫻井英博、持丸真里、増川一．

光生物的水素生産研究開発の再評価の提案－再生可能なエネルギー源の創設を目指して－．水素エネルギーシステム29: 49-55 (2000)

櫻井英博．

生物を利用した光－水素発生系．報告書「人工光合成の開発動向」(日本エネルギー学会) pp. 3-9 (1997)

H. Sakurai, H. Masukawa, K. Inoue,

A Preliminary Survey of the Economical Viability of Large-scale Photobiological Hydrogen Production Utilizing Mariculture-raised Cyanobacteria, in “Handbook on Cyanobacteria: Biochemistry, Biotechnology and Applications” （Gault P.M and Marler, H.J. eds）. Nova Science Publisher (2009, in press)

H. Sakurai, H. Masukawa, Xh. Zhang, H. Ikeda, K. Inoue,

Improvement of nitrogenase-based photobiological hydrogen production by cyanobacteria by gene engineering – hydrogenase and homocitrate synthase. In Photosynthesis. Energy of Sun, J. F. Allen et al. (eds.), pp. 1315-1318. Springer Dordrecht. (2008)

H. Sakurai, H. Masukawa,

Invited review:　Promoting R & D in photobiological hydrogen Production utilizing mariculture-raised cyanobacteria. Mar. Biotechnol. 9: 128-145 (2007)　（他）

[論文]

H. Masukawa, Xh. Zhang, E. Yamazaki, S. Iwata, K. Nakamura, M. Mochimaru, K. Inoue, H. Sakurai,

Survey of the distribution of different types of nitrogenases and hydrogenases in heterocyst-forming cyanobactera. Mar. Biotechnol. 11: 397-409 (2009)

H. Masukawa, K. Inoue, H. Sakurai,

Effects of disruption of homocitrate synthase genes on photobiological hydrogen production and nitrogenase of Nostoc sp. PCC 7120, Appl. Env. Microbiol. 73: 7562-7570 (2007)

F. Yoshino, H. Ikeda, H. Masukawa, H. Sakurai,

High photobiological hydrogen production activity of a Nostoc sp. PCC 7422 uptake hydrogenase-deficient mutant with high nitrogenase activity. Mar. Biotechnol. 9: 101-112 (2007)

F. Yoshino, M. Wakai, H. Masukawa, M. Mochimaru, H. Sakurai.

Photobiological hydrogen production of hydrogenase mutants of heterocystous cyanobacteria. In Photosynthesis: Fundamental Aspects toGlobal Perspectives. (A. van der Est and D. Bruce, eds.). pp. 1087-1089. Allen Press Inc. Lawrence USA (2005)

M. Wakai, S. Dawar, H. Masukawa, H. Sakurai.

Constrution of hydrogenase maturation gene mutants from Anabaena sp. PCC 7120 for improvement of photobiological hydrogen production. In Photosynthesis: Fundamental Aspects toGlobal Perspectives (A. van der Est and D. Bruce, eds.). Allen Press Inc. Lawrence USA, (2005)

Masukawa, M. Mochimaru, H. Sakurai.

Disruption of uptake hydrogenase gene, but not of bi-directional hydrogense gene leads to enhanced photobiological hydrogen production by the nitrogenase-fixing cyanobacterium Anabaena sp. PCC 7120. Appl. Microbiol. Biotechnol. 58: 618-624 (2002)　（他）

[植物生理学、光合成関係]

（桜井英博、柴岡弘郎、芦原坦、高橋陽介、共著）

「植物生理学概論」培風館(2008.10)　　　(リンク書評)（G1-L2-M2b2-N）

Ogawa T., Furusawa, T., Nomura, R., Seo, D., Hosoda-Matsuda, N., Sakurai, H., Inoue, K. (2008)

SoxAX binding protein, a novel component of the thiosulfate-oxidizing multienzyme system in the green sulfur bacterium Chlorobaculum tepidum. J. Bacterol.190: 6097-6110

Seo D, Kamino K, Inoue K,　Sakurai H. (2004)

Purification and characterization of ferredoxin-NADP(+) reductase encoded by Bacillus subtilis yumC. Arch. Microbiol. 182: Pages: 80-89

Mezzetti A, Seo D, Leibl W, Sakurai, H., Breton, J. (2003)

Time-resolved step-scan FTIR investigation on the primary donor of the reaction center from the green sulfur bacterium Chlorobium tepidum. Photosynth. Res. 75: 161-169

Seo D, Sakurai H (2002)

Purification and characterization of ferredoxin-NAD(P)(+) reductase from the green sulfur bacterium Chlorobium tepidum. Biochim. Biophys. Acta 1597: 123-132

Itoh M, Seo D, Sakurai H, Setif P (2002)

Kinetics of electron transfer between soluble cytochrome c-554 and purified reaction center complex from the green sulfur bacterium Chlorobium tepidum. Photosynth. Res. 71: 125-135

Setif P, Seo D, Sakurai H (2001)

Photoreduction and reoxidation of the three iron-sulfur clusters of reaction centers of green sulfur bacteria. Biophys. J. 81: 1208-1219

Seo D, Tomioka A, Kusumoto N, Kamo M, Enami I, Sakurai H (2001)

Purification of ferredoxins and their reaction with purified reaction center complex from the green sulfur bacterium Chlorobium tepidum. BBA-BIOENERGETICS Volume: 1503 Issue: 3 Pages: 377-384

Kusumoto N, Setif P, Brettel K, et al.(1999)

Electron transfer kinetics in purified reaction centers from the green sulfur bacterium Chlorobium tepidum studied by multiple-flash excitation. BIOCHEMISTRY Volume: 38 Issue: 37 Pages: 12124-12137

Noguchi T, Kusumoto N, Inoue Y, et al.(1996)

Electronic and vibrational structure of the radical cation of P-840 in the putative homodimeric reaction center from Chlorobium tepidum as studied by FTIR spectroscopy. BIOCHEMISTRY Volume: 35 Issue: 48 Pages: 15428-15435

Sakurai H, Kusumoto N, Inoue K,(1996)

Function of the reaction center of green sulfur bacteria. PHOTOCHEMISTRY AND PHOTOBIOLOGY Volume: 64 Issue: 1 Pages: 5-13

KUSUMOTO N, INOUE K, SAKURAI H,(1995)

SPECTROSCOPIC STUDIES OF BOUND CYTOCHROME-C AND AN 　IRON-SULFUR　CENTER　 IN A PURIFIED REACTION-CENTER COMPLEX FROM THE GREEN SULFUR BACTERIUM CHLOROBIUM-TEPIDUM. PHOTOSYNTHESIS RESEARCH Volume: 43 Issue: 2 Pages: 107-112

KUSUMOTO N, INOUE K, NASU H, et al. (1994)

PREPARATION OF A PHOTOACTIVE REACTION-CENTER COMPLEX CONTAINING 　PHOTO-REDUCIBLE　FE-S　CENTERＳ　AND PHOTOOXIDIZABLE CYTOCHROME-C FROM THE GREEN SULFUR BACTERIUM CHLOROBIUM-TEPIDUM. PLANT AND CELL PHYSIOLOGY Volume: 35 Issue: 1 Pages: 17-25

FURIHATA T, SUZUKI M, SAKURAI H, ()1992

KINETIC CHARACTERIZATION OF 2 PHOSPHATE-UPTAKE SYSTEMS WITH DIFFERENT AFFINITIES IN SUSPENSION-CULTURED CATHARANTHUS-ROSEUS PROTOPLASTS. PLANT AND CELL PHYSIOLOGY Volume: 33 Issue: 8 Pages: 1151-1157

Mochimaru M, Sakurai H, (1997)

Three kinds of binding site for tentoxin on isolated chloroplast coupling factor 1. FEBS LETTERS Volume: 419 Issue: 1 Pages: 23-26

SAKURAI H, SHINOHARA K, HISABORI T, et al. (1981)

ENHANCEMENT OF ADENOSINE-TRIPHOSPHATASE ACTIVITY OF PURIFIED CHLOROPLAST COUPLING FACTOR-I IN AN AQUEOUS ORGANIC-SOLVENT. JOURNAL OF BIOCHEMISTRY Volume: 90 Issue: 1 Pages: 95-102

FUJII T, YOKOYAMA E, INOUE K, et al. (1990)

THE SITES OF ELECTRON DONATION OF PHOTOSYSTEM-I TO METHYL VIOLOGEN. BIOCHIMICA ET BIOPHYSICA ACTA Volume: 1015 Issue: 1 Pages: 41-48

INOUE K, FUJII T, YOKOYAMA E, et al. (1989)

THE PHOTOINHIBITION SITE OF PHOTOSYSTEM-I IN ISOLATED-CHLOROPLASTS UNDER EXTREMELY REDUCING CONDITIONS. PLANT AND CELL PHYSIOLOGY Volume: 30 Issue: 1 Pages: 65-71

KOJIMA Y, NIINOMI Y, TSUBOI S, et al. (1987)

DESTRUCTION OF PHOTOSYSTEM-I IRON-SULFUR CENTERS OF SPINACH AND ANACYSTIS-NIDULANS BY MERCURIALS. BOTANICAL MAGAZINE-TOKYO Volume: 100 Issue: 1059 Pages: 243-253

INOUE K, SAKURAI H, HIYAMA T, (1986)

PHOTOINACTIVATION SITES OF PHOTOSYSTEM-I IN ISOLATED-CHLOROPLASTS. PLANT AND CELL PHYSIOLOGY Volume: 27 Issue: 6 Pages: 961-968

SAKURAI H, PIETRO AS, (1985)

ASSOCIATION OF FE-S CENTER(S) WITH THE LARGE SUBUNIT(S) OF PHOTOSYSTEM-I PARTICLES. JOURNAL OF BIOCHEMISTRY Volume: 98 Issue: 1 Pages: 69-76

H. Sakurai,　T. Ogawa, T. Furusawa, R. Nomura, M. Shiga, D. Seo, N. Hosoya-Matsuda, K. Inoue, (2009.3.18)

Biochemical studies of the components of thiosulfate oxidizing system in the green sulfur bacterium Chlorobaculum tepidum. EMBO-FEMS Workshop on Microbial Sulphur Metabolism　（15-18 March 2009,　Tomar, Portugal）

メールアドレス

sakurai(a)waseda.jp
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